Application of phototherapy for the healing
of the navels of neonatal dairy calves

Abstract

The present work evaluated the effects of LED light irradiation on the healing of the navels of neonatal dairy calves. Fifty-seven neonatal calves were divided into two groups. Animals had their umbilical stumps immersed in 10% iodine tincture for 60 s, and this process was repeated every 24 h for three consecutive days.The 29 animals in the first group did not receive LED therapy. The 28 animals in the second group received LED light irradiation at 640 nm with 300 mW power, 46.8 J/cm2 energy density, 60 s irradiation time, and 0.385 cm2 spot size. The animals were irradiated at four points (46.8 J/cm2 per point) evenly distributed around the insertion site of the umbilical stump every 24 h for three consecutive days. Irradiation with LED light was applied before the umbilical stumps were immersed in the iodine solution. The time after birth at which the umbilical stump fell off of each calf was noted. The umbilical stumps of all animals fell off by the 25th day of age. After the umbilical stump fell off, the healing of the remnant wound was followed up to the 30th day after birth. The area of the wound was measured on the 15th, 20th, and 25th day after birth using digital photographs and computer-assisted area measurements. A two-tailed unpaired t test was applied to analyze the falling off the umbilical stump, whereas a Kruskal- Wallis one-way ANOVA test with a Dunn’s multiple comparison test was used for the wound size evolution. GraphPad Prisma 5.0® and GraphPad StatMate 2.00® were used for the statistical analysis. The results revealed that phototherapy hastened the falling off the umbilical stump, accelerated navel healing, and reduced the mortality rate in newborn calves. Therefore, this study introduced a preventive and adjuvant after birth treatment that proved to be effective in reducing the incidences of omphalitis and newborn mortality.

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O uso da fototerapia no controle do edema no pós operatório de cirurgias estéticas

Ledterapia no controle da inflamação

Estudo para tratamento de Úlcera por pressão através da Fototerapia com Led.

Avaliação dos efeitos do laser de baixa potência em úlceras utilizando a fotogrametria e índice de retração

Effect of photodynamic therapy on the healing of cutaneous third-degree-burn

The aim of this study was to conduct a histological assessment of the effect of photodynamic therapy (PDT) on the repairing of third-degree-burn wounds made on the backs of rats with a heated scalpel. Ninety-six rats were divided into groups: G1, control (n=24), cold scalpel; G2, burned, heated scalpel (n=24); G3, low-level laser therapy (LLLT) (n=24), on burns; and G4, photodynamic therapy (PDT) (n=24), toluidine-O blue (100 μg/ml) and LLLT treatment on burns. The laser (685 nm) was applied in continuous mode, 50 mW, 4.5 J/cm², contact mode at nine points (9 s/point). Eight animals in each group were killed at 3 days, 7 days or 14 days after surgery, and tissue specimens containing the whole wound-ed area were removed and processed for histological analysis; the results were statistically analyzed with Kruskal–Wallis and Dunn’s tests (P<0.05). The results demonstrated significant differences between G2 and G3, and between G2 and G4, at both 3 days and 7 days, with regard to acute inflammation scores; G1 and G2 showed significant differences when compared with G4 at 3 days, with regard to neo-angiogenesis scores; G1 and G2 were statistically different from G3 and G4 at both 3 days and 7 days, with regard to re-epithelization scores; G2 showed statistically significant differences when compared with G3 and G4 with regard to collagen fiber scores at 7 days. LLLT and PDT acted as a biostimulating coadjuvant agent, balancing the undesirable effect of the burn on the wound healing process, acting mainly in the early healing stages, hastening inflammation and increasing collagen deposition.

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Effect of NASA Light-emitting Diode Irradiation on wound healing

Abstract:

Objective: The purpose of this study was to assess the effects of hyperbaric oxygen (HBO) and near-infrared light therapy on wound healing. Background Data: Light-emitting diodes (LED), originally developed for NASA plant growth experiments in space show promise for delivering light deep into tissues of the body to promote wound healing and human tissue growth. In this paper, we review and present our new data of LED treatment on cells grown in culture, on ischemic and diabetic wounds in rat models, and on acute and chronic wounds in humans. Materials and Methods: In vitro and in vivo (animal and human) studies utilized a variety of LED wavelength, power intensity, and energy density parameters to begin to identify conditions for each biological tissue that are optimal for biostimulation. Results: LED produced in vitro increases of cell growth of 140–200% in mouse-derived fibroblasts, rat-derived osteoblasts, and rat-derived skeletal muscle cells, and increases in growth of 155–171% of normal human epithelial cells. Wound size decreased up to 36% in conjunction with HBO in ischemic rat models. LED produced improvement of greater than 40% in musculoskeletal training injuries in Navy SEAL team members, and decreased wound healing time in crew members aboard a U.S. Naval submarine. LED produced a 47% reduction in pain of children suffering from oral mucositis. Conclusion: We believe that the use of NASA LED for light therapy alone, and in conjunction with hyperbaric oxygen, will greatly enhance the natural wound healing process, and more quickly return the patient to a preinjury/illness level of activity. This work is supported and managed through the NASA Marshall Space Flight Center–SBIR Program.

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Avaliação dos efeitos do LED na cicatrização de feridas cutâneas em ratos wistar

A fisioterapia pode ter importante papel na cicatrização, já que possui recursos terapêuticos capazes de acelerar este processo. Uma opção de tratamento é a fototerapia, e o LED (Light emitting diode) está sendo muito utilizado. Este estudo tem como objetivo avaliar o efeito do LED na cicatrização de feridas cutâneas em ratos Wistar. Utilizou-se uma amostra de 29 ratos do sexo masculino divididos em três grupos, sendo um controle, um tratado com LED verde e o último com o LED vermelho. Após 24 horas da realização das incisões cirúrgicas, foi utilizado o LED nos dois grupos por 6 minutos. Avaliou-se, em nível histológico, a reação inflamatória e o processo de reparo. O resultado demonstrou que o tecido de granulação mostrou-se mais desenvolvido nos grupos irradiados do que no grupo controle e a quantidade de células inflamatórias crônicas (monócitos, macrófagos, linfócitos e plasmócitos) predominaram com a fototerapia verde. A epitelização nas margens da ferida e a formação de cicatrizes com melhor qualidade ocorreram com o LED vermelho (620-630nm), onde ocorreu maior deposição de colágeno. Entretanto, a fototerapia não colimada de 620-630nm (vermelho) provocou melhores efeitos antiinflamatórios. Diante destas respostas com fototerapia não colimada verde (515-525nm) e vermelha (620-630nm), podemos concluir que é possível o uso destes comprimentos de onda com benefícios, preponderantemente com LED vermelho.

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Light-emitting Diode phototherapy at 630  3 nm increases local levels of skin-homing T-cells in human subjects

Background and aims: Red light phototherapy with laser sources has been used successfully for a number of indications. A new generation of quasimonochromatic 630  3 nm light-emitting diode (LED) systems has recently been yielding good results for the same indications, but no study has examined changes in visible red light irradiated skin at an immunological level. This study was thus designed to examine changes in skin-homing T-cell
levels induced in normal human skin by visible red LED energy.

Subjects and methods: Six adult male volunteers (3548 years old) who satisfied all study criteria had the skin over the lateral aspect of the leg irradiated once per week for 8 weeks with a visible red (630  3 nm) LED-based system, with irradiance of 105 mcm², 15 minutes session, and a radiant flux of 94 Jcm². Skin biopsies were performed after the eighth treatment session, and cultures were prepared to assay the type and quantity of skin-homing T-cells using qualitative and quantitative polymerase chain reaction (PCR) techniques. Ultrastructural changes were also assessed with transmission electron microscopy. 

Results: Transmission electron microscopy revealed mild fibroplastic changes in fibroblasts, with no acute inflammatory changes throughout the treatment session. Qualitative PCR showed the presence of both Th-1 and Th-2 T-cells, and quantitative PCR showed an increase in the numbers of both types of skin-homing T-cells, much more so for Th-2 than for Th-1.

Conclusions: Visible red LED irradiation appears to activate the skin-homing immune system.

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The use of hyperbaric oxygen and LEDtherapy in diabetic foot.